Antibiotic Susceptibility Pattern of Gram Negative Bacteria Isolated From Student’s Urine

ABSTRACT

This study was carried out in order to determine the antibiotic susceptibility pattern of gram negative bacteria isolated from student’s urine. One hundred (100) midstream urine samples from students of Ibrahim Badamasi Babangida University were obtained and investigated. The samples were examined microscopically and cultured on MaCconkey agar and blood agar. Disk diffusion method was used for antibiotic susceptibility testing. 49 significant growth yields out of the 100 cultured urine sample, the isolates were further subjected to biochemical tests for possible identification. The bacteria identified were: Escherichia coli 22(44.89%), Pseudomonas sp 4(8.16%), Proteus sp 9(18.36%), Klebsiella sp 10(20.40%), Salmonella sp 4(8.16%). Escherichia coli being the most predominant isolates in the urine samples.
The isolates were very sensitive to Ciprofloxacin, Tarivid, sparfloxacin at (80%) and Gentamicin, Pefloxacin, Augmentin, Streptomycin, Amoxicin and Chloramphenicol at (60%) while the most resistant antibiotics to all isolates were Cotrimozole and Augmentin at (60%) level. E. coli were 100% sensitive to all antibiotics used followed by Klebsiella sp (70%) and Pseudomonas sp (60), while the most resistant isolates to the various antibiotics were Salmonella sp and Proteus sp (30%).
The result of chi-square test of association revealed that the age of the students, Antibiotic usage, History of urinary tract infection and source of water are statistically significant to the occurrence of bacteria in the urine (P < 0.01). Sex was found to be statistically in significant to the occurrence of bacteria in the urine (P > 0.05). It is very important for the students to practice good hygiene and hygienic handling of foods substances to reduce fecal contamination in order to avoid exposure to E. coli and other possible bacteria.